Introduction to Flow Cytometry
In this seminar we will demystify the cytometer through discussion on how they work and discuss best practices in sample preparation/controls.
Intro to Flow Cytometry Session outline
-Instrumentation (what are the key components that make cytometers work)
-Pitfalls of sample preparation – how do I maximize my signal to noise
-Do I need controls? (Yes, several! We will discuss the different types)
-Virtual orientation of the instruments in the flow core
-How do I select the instrument for my hands-on training?
-What do I need to prepare for the hands-on training?
-Rules of the Flow Core
-Short comprehension Quiz
-Resources to further your learning

Your flow cytometry training journey:
o   Theory – Intro to Flow Cytometry –in class session
o   Protocol/sample preparation/panel design consultations
o   Hands-on training (samples required)
o   Data analysis (FlowJo)
o   Figure preparation for presentations/thesis/manuscript

Reference Materials
Andrea Cossarizza , Hyun-Dong Chang , Andreas Radbruch , Sergio Abrignani et al. Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition) Eur J Immunol 2021 Dec;51(12):2708-3145
Andrew Reichard, Kewal Asosingh. Best Practices for Preparing a Single Cell Suspension from Solid Tissues for Flow Cytometry. Cytometry A 2019 Feb;95(2):219-226.
Brooks HL, de Castro Brás LE, Brunt KR, et al. Guidelines on antibody use in physiology research. Am J Physiol Renal Physiol. 2024 Mar 1;326(3):F511-F533.
Ruud Hulspas. Titration of Fluorochrome-Conjugated Antibodies for Labeling Cell Surface Markers on Live Cells. Current Protocols in Cytometry 6.29.1-6.29.9, October 2010
Burn OK, Mair F, Ferrer-Font L. Combinatorial antibody titrations for high-parameter flow cytometry. Cytometry A. 2024 May;105(5):388-393.
Zofia Maciorowski, Pratip K Chattopadhyay, Paresh Jain. Basic Multicolor Flow Cytometry Curr Protoc Immunol 2017 Apr 3:117:5.4.1-5.4.38.
Julie Demaret, Morgane Gossez, Fabienne Venet, Guillaume Monneret. Intracellular Flow Cytometry Improvements in Clinical Studies. Methods Mol Biol 2017:1524:315-327.

Login to Register Back to list
  • Back to top