Thursday, February 2nd
CETS: Droplet Digital PCR Technology and Applications
1:30pm - 2:30pm
Speaker: Dr. Claude Lachance, Applications Scientist (Bio-Rad Canada)
Droplet Digital PCR is a unique technology that enables absolute quantification of nucleic acid target sequences without the need for standard curves. When considering complex matrices (FFPE, liquid biopsies, environmental samples etc.), Droplet Digital™ PCR offers orders of magnitude higher precision and sensitivity than standard qPCR. Our newly acquired Bio-Rad QX200™ Droplet Digital PCR system (CFI) overcomes the previous lack of scalable and practical technologies for digital PCR implementations and has led to several breakthroughs in the fields of cancer, infectious diseases, and environmental sciences.
This talk will provide a technical overview of the QX200™ Droplet Digital PCR (ddPCR) system and its workflow. Data from key applications will be presented, highlighting unique capabilities and higher precision and sensitivity in detection compared to real-time PCR. In addition, single cell sequencing workflow with droplet technology will be introduced in anticipation of the acquisition of an Illumina NGS.
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